mjsa.01.2026.80.83
AI-DRIVEN MICROALGAL BIOETHANOL PRODUCTION: OPTIMIZING CULTIVATION, STRAIN ENGINEERING AND CONVERSION PROCESSES
Journal: Malaysian Journal of Sustainable Agriculture (MJSA)
Author: Mudasir Hussaint, Inayat Ali, Bisma Jabbar, Sara Ikram
This is an open access article distributed under the Creative Commons Attribution License CC BY 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
DOI: 10.26480/mjsa.01.2026.80.83
ABSTRACT

and high-carbohydrate content, although current ethanol yields remain modest. This review article analyzes
how artificial intelligence can substantially enhance microalgal bioethanol production across all stages, from
cultivation to bioconversion. We structured Al applications to enhance cultivation parameters such as light,
CO₂, and nutrients through real-time sensor monitoring and machine-learning control, which have increased
algal biomass productivity by approximately 15-50% in preliminary trials. Discuss Al-guided strain selection
and metabolic engineering strategies that leverage omics data and predictive modeling to identify high-
carbohydrate phenotypes, with recent work proposing up to 30-40% improvements in product yield via Al-
optimized strain design. Downstream, Al-driven process enhancement in pretreatment and fermentation can
improve sugar release and fermentation effectiveness, for instance, by forecasting optimal pretreatment
conditions and dynamically maintaining fermentation parameters to maximize ethanol titer. A unified digital
twin framework is proposed as a future paradigm in which the digital counterpart of the algal biorefinery
continuously learns and optimizes the entire process, from photobioreactor to fermentation, in silico. While
Al offers significant gains in efficiency and product yield, we note that data insufficiency, model
generalizability and scaling issues remain challenges. Tackling these issues through interdisciplinary
partnership and data-sharing will be important. Overall, Al integration can accelerate micro-algal bioethanol
development, making production more versatile, productive and eco-friendly.
| Pages | 80-83 |
| Year | 2026 |
| Issue | 1 |
| Volume | 10 |


