Malaysian Journal of Sustainable Agriculture (MJSA)

AI-DRIVEN MICROALGAL BIOETHANOL PRODUCTION: OPTIMIZING CULTIVATION, STRAIN ENGINEERING AND CONVERSION PROCESSES

mjsa.01.2026.80.83

AI-DRIVEN MICROALGAL BIOETHANOL PRODUCTION: OPTIMIZING CULTIVATION, STRAIN ENGINEERING AND CONVERSION PROCESSES

Journal: Malaysian Journal of Sustainable Agriculture (MJSA)

Author: Mudasir Hussaint, Inayat Ali, Bisma Jabbar, Sara Ikram

This is an open access article distributed under the Creative Commons Attribution License CC BY 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

DOI: 10.26480/mjsa.01.2026.80.83

ABSTRACT

Microalgae are a promising third-generation feedstock for bioethanol production due to their rapid growth
and high-carbohydrate content, although current ethanol yields remain modest. This review article analyzes
how artificial intelligence can substantially enhance microalgal bioethanol production across all stages, from
cultivation to bioconversion. We structured Al applications to enhance cultivation parameters such as light,
CO₂, and nutrients through real-time sensor monitoring and machine-learning control, which have increased
algal biomass productivity by approximately 15-50% in preliminary trials. Discuss Al-guided strain selection
and metabolic engineering strategies that leverage omics data and predictive modeling to identify high-
carbohydrate phenotypes, with recent work proposing up to 30-40% improvements in product yield via Al-
optimized strain design. Downstream, Al-driven process enhancement in pretreatment and fermentation can
improve sugar release and fermentation effectiveness, for instance, by forecasting optimal pretreatment
conditions and dynamically maintaining fermentation parameters to maximize ethanol titer. A unified digital
twin framework is proposed as a future paradigm in which the digital counterpart of the algal biorefinery
continuously learns and optimizes the entire process, from photobioreactor to fermentation, in silico. While
Al offers significant gains in efficiency and product yield, we note that data insufficiency, model
generalizability and scaling issues remain challenges. Tackling these issues through interdisciplinary
partnership and data-sharing will be important. Overall, Al integration can accelerate micro-algal bioethanol
development, making production more versatile, productive and eco-friendly.
Pages 80-83
Year 2026
Issue 1
Volume 10

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mjsa.02.2026.103.114

IMPACT OF CLIMATE CHANGE ON SOIL-WATER QUALITY, FOOD, AND NUTRITIONAL SECURITY IN THE COASTAL AREAS OF BANGLADESH: A CLIMATE CRISIS MANAGEMENT-BASED REVIEW TO ACHIEVE SDGS

Journal: Malaysian Journal of Sustainable Agriculture (MJSA)
Author: Prattaya Chakrabortty, Md. Shamsuzzoha, Md. Shariful Isla”, Md. Anwarul Abedin, Md. Sujahangir Kabir Sarkar, Mst Moriom Khatuns, Rajib Shaw

This is an open access article distributed under the Creative Commons Attribution License CC BY 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

DOI: 10.26480/mjsa.02.2026.103.114

ABSTRACT

Climate change has already received global attention as it is threatening vulnerable communities, sectors, regions, and countries. Bangladesh, especially in coastal areas, is not of exception, because of witnessed the adverse effects of climate change. Increasing industrialization, urbanization, and the greenhouse effect are affecting the climate and causing scenarios like increased soil and water salinity, deforestation, surface water contamination, reduced groundwater level, heavy metal pollution in soil, water, and food, malnutrition, temperature rise, late precipitation, and cropping season shifting etc. These adverse effects affect people’s well-being, particularly vulnerable communities, agricultural production, and natural biodiversity. This paper is focused on reviewing literature about the impact of climate change on the soil-water, food, and nutrition sectors in coastal areas of Bangladesh. According to this study, climate change has made the coastal region of Bangladesh even more vulnerable to problems with nutrition, food security, and soil-water quality. Sustainable farming methods, climate-smart agriculture (CSA), and creative approaches to water management, livelihood diversification, and enhancing emergency and humanitarian responses should be the main focuses of climate crisis solutions. Thus, coordinating these initiatives with the management of the climate crisis will enhance sustainable development goals (SDGs) and promote long-term prosperity and food security for the coastal community.

Pages 103-114
Year 2026
Issue 2
Volume 10

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mjsa.02.2026.96.102

POTENTIAL USE OF PRICKLY PEAR (OPUNTIA FİCUS-İNDİCA) AS A FODDER PLANT: AN AGRICULTURAL AND SOIL PRODUCTİVİTY ASSESSMENT ON THE ADANA PLAİN EXAMPLE

Journal: Malaysian Journal of Sustainable Agriculture (MJSA)
Author: Adem Erol, Hamdi Ayyıldız

This is an open access article distributed under the Creative Commons Attribution License CC BY 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

DOI: 10.26480/mjsa.02.2026.96.102

ABSTRACT

The aim of this study was to explore the potential of prickly pear Opuntia ficus-indica as a sustainable fodder crop and soil ameliorant in semi-arid conditions in Adana Plain, southern Türkiye. Followed by high forage productivity and ecological restoration, O. ficus-indica is not only known for its Crassulacean Acid Metabolism (CAM) but also for its impressive resilience to drought, heat, and low soils.For green forage yield, field experiments in 2023 showed an average yield of 18.2t/da/year or about 182t/ha/year with a dry matter and crude protein contents of about 12.4 and 5.2%, respectively. The in vitro dry matter digestibility (IVDMD) was 57.8 and as such is confirmed to be a significant moisture content, highly digestible forage for integrated livestock systems. Meanwhile, a post-harvest soil analysis revealed significant gains in the ecology, with the amount of organic matter increased by 6.3%, nitrogen enriched approximately 8.7 times and pH between stable, this contributed to the composite Soil Fertility Change Index (SFCI) values from 1.23 to 1.52 for all plots. The findings were further strengthened through multivariate analyses, which added depth and interpretive power. Using k-means clustering, three distinct performance groups were identified, with Group A having the highest average productivity and soil fertility indicators. Pearson correlation analysis indicated strong correlations with soil organic matter (r = 0.8) nitrogen content (r = 0.72) and biomass yield (r = 0.78) while, separated by Principal Component Analysis (PCA), Factor 1 correlated with soil quality, and Factor 2 correlated to forage quality. Green forage yield also was a bridge variable between the two factors.
Such results add information to O. ficus-indica as sustainable feedstuff and soil restoration tool. With its low-input needs, high adaptation potential and multifunctional role as a food source, ingredient and industrial crop, it is now positioned as a strategic crop under climate-resilient Mediterranean agriculture (and alike). This study adds a strong theoretical foundation for inserting 0. ficus-indica in global warming adaptation strategies of restoration and feed security at the regional level.

Pages 96-102
Year 2026
Issue 2
Volume 10

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mjsa.02.2026.91.95

IOT-BASED SMART FARMING SYSTEM FOR NON-DESTRUCTIVE REAL-TIME SOIL MONITORING OF SORGHUM CROP

Journal: Malaysian Journal of Sustainable Agriculture (MJSA)
Author: Waqas Safdar, Muhammad Hamza Abbas, Hammad Shahab, Shahzad Hussain, Danish Akram, Muhammad Mohsin Waqas

This is an open access article distributed under the Creative Commons Attribution License CC BY 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

DOI: 10.26480/mjsa.02.2026.91.95

ABSTRACT

As a solution to challenges associated with global agricultural productivity, Sustainable Development Goals (SDGs) focus on smart agricultural practices in order to support the global population of 9.7 billion people projected to exist in 2050. This study presents an loT-based smart system to monitor soil nutrients and prescribe fertilizers on real time basis, which measures eight major soil parameters namely moisture, salinity, temperature, electrical conductivity (EC), pH, nitrogen, phosphorus, and potassium. The real-time monitoring capability of the system was confirmed by field experiments conducted on the vegetative stage of the sorghum crops at Rahim Yar Khan, in Pakistan. 10 samples of the soil were taken using the proposed loT device at the depth of 0-6 inches in different locations within the sorghum field. The system was proved to be quicker than the manual soil testing technique in measurements, therefore providing a more precise, real-time observation of soil fertility, decreasing human error, and providing a timely and data-driven decision-making to optimize crop management.

Pages 91-95
Year 2026
Issue 2
Volume 10

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mjsa.02.2026.84.90

DOUBLE-ROW ARRANGEMENT INCREASES COMPETITIVENESS IN SORGHUM-GREEN GRAM INTERCROPPING SYSTEM IN DRYLAND AREAS OF KENYA

Journal: Malaysian Journal of Sustainable Agriculture (MJSA)
Author: Bernard M. Yumbya

This is an open access article distributed under the Creative Commons Attribution License CC BY 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

DOI: 10.26480/mjsa.02.2026.84.90

ABSTRACT

Recurrent droughts have threatened crop yields and livelihoods of many small-scale farmers in dryland areas of Kenya. An evaluation of sorghum [Sorghum bicolor (L) Moench) and green gram [(Vigna radiata (L.) Wilezek] intercropping was conducted to determine the yield and competitiveness among the green gram varieties and crop arrangements. Two field experiments were performed simultaneously during the 2022 short rain season in two dryland areas of southeastern Kenya. Four green gram varieties (N26, KS20, Biashara, and Karembo) and three crop arrangements (sole crop, double row, and single row) and control of both sole crop green gram and sorghum were used. Treatments were placed in a randomized complete block design with a split-plot arrangement where a crop arrangement system formed the main plots while the green gram variety assumed the subplots and was replicated three times. Data collected were soil fertility status at the start of the trials, nodulation, plant height, and yield of green gram and sorghum. Competition ratio was used to assess the intercropping systems. Green gram variety N26 outperformed the other varieties, irrespective of crop arrangement. Competition ratios (CR) between the two crops showed that the CR of green gram was >1, thus legume(s) were more aggressive in affecting the growth of sorghum. The study findings indicate that green gram was more aggressive than sorghum and the double row planting of N26 enhances competitiveness and productivity of sorghum-green gram intercropping systems in dryland areas. This crop arrangement can improve land use efficiency, increase grain yields, and contribute to improved food security in arid and semi-arid regions of Kenya. Further studies are proposed to screen for green gram varieties that are compatible with sorghum to provide optimal intercropping productivity in different agro ecological zones.

Pages 84-90
Year 2026
Issue 2
Volume 10

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mjsa.01.2026.71.79

YIELD RESPONSE AND N USE EFFICIENCY OF ZERO-TILLED CAPSICUM TO NITROGEN VARIATIONS IN THE COASTAL SOIL OF GANGES DELTA

Journal: Malaysian Journal of Sustainable Agriculture (MJSA)

Author: Apshory Fatama Ahamed, Md. Sarwar Jahan, Bipro Roy and Bidhan Chandro Sarker

This is an open access article distributed under the Creative Commons Attribution License CC BY 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

DOI: 10.26480/mjsa.01.2026.71.79

ABSTRACT

Nitrogen (N) is the most vital component for the productivity and profitability of crops, particularly in N-deficient soil. The use efficiency of N mostly depends on its judicious application and availability. Thus, the experiment was conducted in the farmer’s field of the southwestern coastal region during winter (2024-25) to investigate the growth, yield response and N use efficiency (NUE) of capsicum to various N rates under zero-tillage conditions. The factorial experiment comprised two capsicum varieties (Astha and BARI Misti Morich-2) and five rates of N (0, 90, 120, 150 and 180 kg ha¹). The experimental design was a factorial randomized complete block design and replicated three times. The individual effect of variety and N showed significant influence on growth, yield and profitability, yet their interaction was not significant. The growth, yield attributes, yield, total soluble solids, profitability, and NUE of Astha were substantially higher than BARI Misti Morich-2, where the yield, net income, and NUE were enhanced by 13%, 43%, and 13%, respectively. Among the N rates, application of N at180 kg ha¹ produced the highest yield, which was increased by 4 to 71% from 150 to 90 kg N ha yet the yield was statistically similar with 150 kg N ha-¹. Total soluble solids were substantially increased with the increased rate of N, but vitamin C content initially increased from 0 to 120 kg N hathen gradually declined with the higher N rates beyond 150 kg hat. The net income declined by 1.09 to 7.35 times from 120 to 90 kg N ha¹ compared to 180 kg N ha¹, yet there was no significant variation between 150 and 180 kg N ha¹ while in control treatment the net income was negative. The NUE was higher in the lower rates and then gradually decreased with the higher rates, where the NUE declined by 3 to 15% from the application of 120 to 180 kg N ha compared to 90 kg N ha¹. The results of this experiment suggested that the Astha variety is suitable with the application of 150 kg N hat under zero tillage conditions in the southwestern coastal soil of Bangladesh.
Pages 71-79
Year 2026
Issue 1
Volume 10

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mjsa.01.2026.62.70

SUSTAINABILITY IMPACT ASSESSMENT CRITERIA OF ORGANIC FOOD SUPPLY CHAINS IN SRI LANKA

Journal: Malaysian Journal of Sustainable Agriculture (MJSA)

Author: Indra Mahakalanda”, M.M.Thiloni S. Marasinghe”, Navaka Navaratne

This is an open access article distributed under the Creative Commons Attribution License CC BY 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

DOI: 10.26480/mjsa.01.2026.69.77

ABSTRACT

The inspiration of this study pertains to the problem of identifying and prioritizing sustainability impact assessment criteria of local organic food supply chains. Scoring models are often regarded as the most promising method of handling this increasingly important sector, but the literature on sustainability assessment concepts is only emerging. Multi-Criteria Decision Analysis based techniques such as Fuzzy Analytic Hierarchy Process (FAHP) appear practically appealing, as a way to develop commercially viable arrangements to develop sustainability assessment decision models. The first phase of our study identifies sustainability assessment criteria for organic food supply chains through literature review and expert panel interviews. Literature identifies environmental sustainability, economic sustainability and societal sustainability as the main criteria and a set of sub-criteria under each main criterion. The second phase develops a FAHP survey questionnaire for criteria analysis and weighting. Sub criteria such as Reduction of Food Waste, Reduction of Impact from Transportation and Emissions and Transparency and Traceability receive highest weights under economic, environmental and social criteria. Our research provides a structured decision-making framework which can be a valuable tool for strategic planning, enabling targeted interventions to improve farmers’ resilience and support the growth of the organic sector in Sri Lanka.
Pages 62-70
Year 2026
Issue 1
Volume 10

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mjsa.01.2026.39.45

REPURPOSING MAIZE STRAW AND CHICKEN DUNG INTO MATURE AND NON-PHYTOTOXIC ORGANIC AMENDMENTS

Journal: Malaysian Journal of Sustainable Agriculture (MJSA)

Author: Khoirunnisaa’ Kamaluddin Aniisah and Osumanu Haruna Ahmed

This is an open access article distributed under the Creative Commons Attribution License CC BY 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

DOI: 10.26480/mjsa.01.2026.39.45

ABSTRACT

Agricultural residues such as maize straw and chicken dung are produced in substantial quantities in Brunei Darussalam, but remain underutilised due to limited waste management and utilisation strategies. This study evaluated whether composting and co-composting these materials could produce mature and non-phytotoxic organic amendments. Accordingly, three organic amendments were produced from high-carbon and high-nitrogen agricultural wastes: (i) maize straw compost (MS), (ii) maize straw-chicken dung co-compost (MS:CD), and (iii) chicken dung compost (CD). Temperature profiles indicated differing thermal dynamics among the materials. Maize straw compost showed modest thermal activity (28.2 °C to 42.1 °C), MS:CD maintained warm-mesophilic conditions (30.4 °C to 39.0 °C), whereas CD reached a near-thermophilic peak of 50.0 °C. All three organic amendments exhibited near-neutral to slightly alkaline pH (7.19 to 7.87), C/N ratios of 11.6 to 13.9, and organic matter contents between 53.1% and 57.5%. Water-extractable aluminium ions were not detected, and available iron ions content remained low (0.012 cmol kg¹ to 0.231 cmol kg 1). Phytotoxicity assessment using a water spinach (Ipomoea aquatica F.) germination test showed no inhibitory effects for any of the organic amendments when compared with the soil-only control. Germination indices exceeded 126%, whereas vigour indices of the organic amendments were significantly higher than those observed in the control treatment. Overall, composting and co-composting maize straw and chicken dung produced mature and non-phytotoxic organic amendments, representing a promising pathway for valorising agricultural residues in Brunei Darussalam. However, further soil-based trials are required to confirm their agronomic performance.
Pages 39-45
Year 2026
Issue 1
Volume 10

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mjsa.01.2026.53.61

PHOSPHORUS AVAILABILITY IN MINERAL ACID SOILS IMPROVES USING SOIL ORGANIC CONDITIONER PRODUCED FROM CHICKEN DUNG

Journal: Malaysian Journal of Sustainable Agriculture (MJSA)

Author: Siti Hadhirah Azlan, Osumanu Haruna Ahmed, Rose Abdullah, Nur Thaqifah Salihah, Syahirah Shahlehi, Kathereen Liew, Mohamed Mujithaba Mohamed Najim, Hassan Ammouneh, and Siti Nur Idayu Matusin

This is an open access article distributed under the Creative Commons Attribution License CC BY 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

DOI: 10.26480/mjsa.01.2026.53.61

ABSTRACT

Brunei Darussalam has long faced challenges associated with low-pH soils, which contribute to low crop productivity. These highly weathered soils are characterized by low nutrient availability and high concentrations of aluminium (Al) and iron (Fe), which strongly bind phosphorus (P) through chelation thus rendering it unavailable to plants. Although the application of inorganic P fertilizers partially alleviates P deficiency, substantial losses occur through leaching, fixation, and immobilization. The use of an organic soil conditioner (OSC) derived from chicken manure represents a practical and sustainable approach for improving soil P availability. An incubation study was conducted to evaluate changes in soil available P and selected soil chemical properties. The experiment was arranged in a Completely Randomized Design (CRD) with five treatments: T1 (0% OSC), T2 (100% OSC), T3 (75% OSC), T4 (50% OSC), and T5 (25% OSC), each with three replicates over a 30-day incubation. The application of OSC increased soil pH and electrical conductivity (EC). Moreover, this approach significantly reduced the soil exchangeable acidity, Al, and Hthrough dissolution and neutralization reactions involving organic anions. Consequently there was an increment of 402.411154.85 ppm available P with the treated soil compared with the control (T1). In addition, the increase in soil organic carbon and organic matter enhanced P availability by promoting nutrient cycling and reducing P fixation. These findings demonstrate that OSC effectively ameliorates soil acidity and enhances P availability, indicating its potential to improve crop productivity on mineral acid soils.
Pages 53-61
Year 2026
Issue 1
Volume 10

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mjsa.01.2026.46.52

COMPARISON OF REFERENCE EVAPOTRANSPIRATION ESTIMATES IN BORNEO FOR IRRIGATION MANAGEMENT IN BRUNΕΙ

Journal: Malaysian Journal of Sustainable Agriculture (MJSA)

Author: Siti Nurzattey Amirah binti Fadilah, Mohamed Mujithaba Mohamed Najim, Hassan Ammouneh, Syahirah binti Haji Shahlehi, Ahmed Osumanu Haruna

This is an open access article distributed under the Creative Commons Attribution License CC BY 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

DOI: 10.26480/mjsa.01.2026.46.52

ABSTRACT

CROPWAT-CLIMWAT recommended Labuan weather station as a climatic proxy for estimating reference evapotranspiration (ET) and crop water requirements in Brunei Darussalam. Labuan is an island hence this data might not provide better estimations of evapotranspiration to the Bruneian conditions. This study evaluates the suitability of Labuan weather data for estimating reference evapotranspiration (ET) in Brunei. Long-term meteorological data from Labuan and 11 other stations across Borneo were compared with data from two Bruneian stations, Pekan Tutong and Sinaut. Statistical analyses, including t-tests of monthly temperature, humidity, wind speed, sunshine hours, and derived ET, revealed that Labuan exhibits a distinctly moderated maritime climate, with significant differences (p < 0.05) in key drivers of evapotranspiration compared to the Bruneian sites. Labuan’s monthly ET, systematically underestimates Bruneian ET,, with deviations exceeding 14% during critical irrigation periods. No single external Borneo station in the CLIMWAT database provided a statistically suitable substitute for the sites in Brunei. It is found that geographic proximity does not guarantee climatic homogeneity for energy-driven variables like ET,, as local topography and continentality create distinct microclimates. It is concluded that the use of Labuan data to estimate ET and then irrigation planning for Brunei either over or underestimate those hence it is recommended to use local meteorological data to the Bruneian conditions.
Pages 46-52
Year 2026
Issue 1
Volume 10

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