Malaysian Journal of Sustainable Agriculture (MJSA)

BIOREMEDIATION OF OIL CONTAMINATED DRILL-CUTTINGS USING DIFFERENT STRAINS OF NATIVE SOIL BACTERIA AND FUNGI FROM THE KURDISTAN REGION OF IRAQ

ABSTRACT

BIOREMEDIATION OF OIL CONTAMINATED DRILL-CUTTINGS USING DIFFERENT STRAINS OF NATIVE SOIL BACTERIA AND FUNGI FROM THE KURDISTAN REGION OF IRAQ

Journal: Malaysian Journal of Sustainable Agriculture (MJSA)
Author: Tablo Abdulrahim Ahmed , Dilshad Ganjo.Ahmed

This is an open access article distributed under the Creative Commons Attribution License CC BY 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

DOI: 10.26480/mjsa.01.2024.20.27

Consortia of 4 bacterial and 4 fungal hydrocarbon-utilizing isolates was analyzed in remediation of water
based oil fields. Bio-augmentation experiments (in situ) were tested in 16 triplicates (excluding the control). The results (after two months of bioremediation) showed that; A consortium of isolates of Pseudomonas
fluorescens-LR134300.1 and Aspergillus fumigatus-KU321562 was able to change the pH from strongly alkaline to almost neutral. A consortium of Kocuria rosea-MK648258 and Aspergillus niger-MK452260 isolates showed high biodegradation and high chloride tolerance. A consortium of Pseudomonas fluorescens LR134300.1 and Penicillium chrysogenum-MK696383.1 isolates showed the highest percentage of total petroleum hydrocarbon (TPH) degradation and could degrade/utilize a range of carbon fractions from C6 to C36. A consortium of isolates of Kocuria rosea-MK648258 and Aspergillus flavus-MH270609.1 showed the highest lead-reducing capacity, while Bacillus subtilies-MK000710 and Penicillium chrysogenum
MK696383-KU321. The consortium extracts mineralized petroleum hydrocarbons as the sole source of energy and carbon, with mineralization rates statistically significant (Pandlt;0.05). The results of the Toxicity Characterization Leaching Procedure (TCLP) test showed that treating the drill cuttings with different consortia of bacterial and fungal strains proved to be a desirable disposal method.
Pages 20-27
Year 2024
Issue 1
Volume 8

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