MOLECULAR ISOLATION, SCREENING AND IDENTIFICATION OF HYDROCARBON DEGRADING FUNGI FROM OIL CONTAMINATED SOILS, KRI, IRAQ
Journal: Malaysian Journal of Sustainable Agriculture (MJSA)
Author: Tablo Abdulrahim Ahmed, Dilshad Ganjo.Ahmed
This is an open access article distributed under the Creative Commons Attribution License CC BY 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
This study aims to isolate and recognize (native/indigenous) hydrocarbon degrading fungal strains from oil-laden soils, at different geographical locations in KRI oilfields, Iraq. The identification was mainly based on macroscopic and microscopic examinations, but due to morphological similarities, which make the identification at species level doubtful in some cases, an attempt was made to use molecular techniques as well, as confirmatory/comparative analysis. The extracted DNA from hypha was used for polymerase chain reaction (PCR) amplification, using specific internal transcribed spacer primer (ITS1/ITS2). PCR product sequencing analyses were compared with the other related sequences in GenBank (NCBI) for molecular evolutionary analyses. Results showed that; out of 68 screened fungal strains; only two genera (i.e. Aspergillus spp. and Penicillium sp.) were identified as the most potent hydrocarbon degrading fungi. DNA sequence analysis confirmed the validity of the species as; Aspergillus fumigatus-KU321562.1; A. flavus-MH270609.1; A. niger-MK452260.1 and Penicillium chrysogenum-MK696383.1.