Okra (Abelmoschus esculentus L.) is one of the important crops grown mainly for its high nutritional and economic significance in the tropic and subtropic. The suitability of radiation from projector for inducing genetic diversity in okra was studied. Okra seeds were exposed to projected radiation at two distances from the projector (10 cm and 30 cm) for two durations (30 minutes and 1 hour), while seeds not exposed to radiations serves as controls for the study. The cetyltrimethylammonium bromide (CTAB) method was applied for the extraction of genomic DNA from young leaves of M1-generation plants while yield of DNA as well as the qualities were quantified using spectrophotometry. Similarly, the level of genetic variability was quantified using PCR amplification with three Start Codon Targeted (SCoT) markers to confirm the level of polymorphisms. Exposed okra seeds to radiation at a distance of 10 cm for 30 minutes significantly enhanced the DNA concentration, purity, and polymorphism, with the SCoT-1 primer detecting 100% polymorphism.When exposure duration was extended further to 1 hour, DNA degradation and reduction in the number of polymorphic bands were observed. Clustering followed genetic differentiation in relation to distance and duration of radiation exposure. The study indicates that projector radiation alters DNA concentration, purity, and create genetic variability in okra, with moderate doses optimizing genetic polymorphism while limiting degradation. These findings reveal that projector radiation is a potential cost-effective agent for generating genetic diversity during okra breeding programs.